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Toward new insights on the white blood cell differential by flow cytometry: A proof of concept study on the sepsis model

Abstract : Background: We sought to evaluate, on a model of sepsis, the clinical relevance of new parameters obtained on a white blood cell (WBC) differential by flow cytometry, implemented in the routine workflow of our hematology laboratory. Methods: A WBC with differential by flow cytometry was done on 459 patients at admission in intensive care unit. They were retrospectively categorized in having (i) infection or not or (ii) a high gravity score (severe sepsis or septic shock) or not. We analyzed by hierarchical clustering, in a multidimensional manner, 50 parameters provided by the flow cytometric platform in place of the standard seven parameters for a standard differential. Results: Our approach allows to discriminate on the basis of a WBC differential (i) infected patients at admission based on a 16 parameter signature, with a concordance rate of 72.7% and a specificity of 79.9% and (ii) patients with high gravity score (septic shock or severe sepsis) at admission with a signature of eight parameters, with a concordance rate of 74.7% and a specificity of 75.9%. Conclusions: This study shows the clinical relevance of an extended WBC differential to obtain by a flow cytometer integrated into a routine hematology laboratory workflow. Development of such approach implicates the redefinition of the WBC differential by integrating new parameters. © 2012 International Clinical Cytometry Society
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https://hal.ehesp.fr/hal-01647781
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Submitted on : Friday, November 24, 2017 - 3:30:15 PM
Last modification on : Wednesday, October 27, 2021 - 3:56:17 PM

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Mikael Roussel, Antoine Gros, Arnaud Gacouin, Nolwenn Le Meur, Yves Le Tulzo, et al.. Toward new insights on the white blood cell differential by flow cytometry: A proof of concept study on the sepsis model. Cytometry Part B: Clinical Cytometry, Wiley, 2012, 82B (6), pp.345 - 352. ⟨10.1002/cyto.b.21027⟩. ⟨hal-01647781⟩

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